Bioinformatics Data Skills. Reproducible and Robust Research with Open Source Tools Vince Buffalo

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Spis treści książki

• Preface
  • The Approach of This Book
  • Why This Book Focuses on Sequencing Data
  • Audience
  • The Difficulty Level of Bioinformatics Data Skills
  • Assumptions This Book Makes
  • Supplementary Material on GitHub
  • Computing Resources and Setup
  • Organization of This Book
  • Code Conventions
  • Conventions Used in This Book
  • Using Code Examples
  • Safari Books Online
  • How to Contact Us
  • Acknowledgments
• I. Ideology: Data Skills for Robust and Reproducible Bioinformatics
• 1. How to Learn Bioinformatics
  • Why Bioinformatics? Biologys Growing Data
  • Learning Data Skills to Learn Bioinformatics
  • New Challenges for Reproducible and Robust Research
  • Reproducible Research
  • Robust Research and the Golden Rule of Bioinformatics
  • Adopting Robust and Reproducible Practices Will Make Your Life Easier, Too
  • Recommendations for Robust Research
    • Pay Attention to Experimental Design
    • Write Code for Humans, Write Data for Computers
    • Let Your Computer Do the Work For You
    • Make Assertions and Be Loud, in Code and in Your Methods
    • Test Code, or Better Yet, Let Code Test Code
    • Use Existing Libraries Whenever Possible
    • Treat Data as Read-Only
    • Spend Time Developing Frequently Used Scripts into Tools
    • Let Data Prove That Its High Quality
  • Recommendations for Reproducible Research
    • Release Your Code and Data
    • Document Everything
    • Make Figures and Statistics the Results of Scripts
    • Use Code as Documentation
  • Continually Improving Your Bioinformatics Data Skills
• II. Prerequisites: Essential Skills for Getting Started with a Bioinformatics Project
• 2. Setting Up and Managing a Bioinformatics Project
  • Project Directories and Directory Structures
  • Project Documentation
  • Use Directories to Divide Up Your Project into Subprojects
  • Organizing Data to Automate File Processing Tasks
  • Markdown for Project Notebooks
    • Markdown Formatting Basics
    • Using Pandoc to Render Markdown to HTML
• 3. Remedial Unix Shell
  • Why Do We Use Unix in Bioinformatics? Modularity and the Unix Philosophy
  • Working with Streams and Redirection
    • Redirecting Standard Out to a File
    • Redirecting Standard Error
    • Using Standard Input Redirection
  • The Almighty Unix Pipe: Speed and Beauty in One
    • Pipes in Action: Creating Simple Programs with Grep and Pipes
    • Combining Pipes and Redirection
    • Even More Redirection: A tee in Your Pipe
  • Managing and Interacting with Processes
    • Background Processes
    • Killing Processes
    • Exit Status: How to Programmatically Tell Whether Your Command Worked
  • Command Substitution
• 4. Working with Remote Machines
  • Connecting to Remote Machines with SSH
  • Quick Authentication with SSH Keys
  • Maintaining Long-Running Jobs with nohup and tmux
    • nohup
  • Working with Remote Machines Through Tmux
    • Installing and Configuring Tmux
    • Creating, Detaching, and Attaching Tmux Sessions
    • Working with Tmux Windows
• 5. Git for Scientists
  • Why Git Is Necessary in Bioinformatics Projects
    • Git Allows You to Keep Snapshots of Your Project
    • Git Helps You Keep Track of Important Changes to Code
    • Git Helps Keep Software Organized and Available After People Leave
  • Installing Git
  • Basic Git: Creating Repositories, Tracking Files, and Staging and Committing Changes
    • Git Setup: Telling Git Who You Are
    • git init and git clone: Creating Repositories
    • Tracking Files in Git: git add and git status Part I
    • Staging Files in Git: git add and git status Part II
    • git commit: Taking a Snapshot of Your Project
    • Seeing File Differences: git diff
    • Seeing Your Commit History: git log
    • Moving and Removing Files: git mv and git rm
    • Telling Git What to Ignore: .gitignore
    • Undoing a Stage: git reset
  • Collaborating with Git: Git Remotes, git push, and git pull
    • Creating a Shared Central Repository with GitHub
    • Authenticating with Git Remotes
    • Connecting with Git Remotes: git remote
    • Pushing Commits to a Remote Repository with git push
    • Pulling Commits from a Remote Repository with git pull
    • Working with Your Collaborators: Pushing and Pulling
    • Merge Conflicts
    • More GitHub Workflows: Forking and Pull Requests
  • Using Git to Make Life Easier: Working with Past Commits
    • Getting Files from the Past: git checkout
    • Stashing Your Changes: git stash
    • More git diff: Comparing Commits and Files
    • Undoing and Editing Commits: git commit --amend
  • Working with Branches
    • Creating and Working with Branches: git branch and git checkout
    • Merging Branches: git merge
    • Branches and Remotes
  • Continuing Your Git Education
• 6. Bioinformatics Data
  • Retrieving Bioinformatics Data
    • Downloading Data with wget and curl
      • wget
      • Curl
    • Rsync and Secure Copy (scp)
  • Data Integrity
    • SHA and MD5 Checksums
  • Looking at Differences Between Data
  • Compressing Data and Working with Compressed Data
    • gzip
    • Working with Gzipped Compressed Files
  • Case Study: Reproducibly Downloading Data
• III. Practice: Bioinformatics Data Skills
• 7. Unix Data Tools
  • Unix Data Tools and the Unix One-Liner Approach: Lessons from Programming Pearls
  • When to Use the Unix Pipeline Approach and How to Use It Safely
  • Inspecting and Manipulating Text Data with Unix Tools
    • Inspecting Data with Head and Tail
    • less
    • Plain-Text Data Summary Information with wc, ls, and awk
    • Working with Column Data with cut and Columns
    • Formatting Tabular Data with column
    • The All-Powerful Grep
    • Decoding Plain-Text Data: hexdump
    • Sorting Plain-Text Data with Sort
    • Finding Unique Values in Uniq
    • Join
    • Text Processing with Awk
    • Bioawk: An Awk for Biological Formats
    • Stream Editing with Sed
  • Advanced Shell Tricks
    • Subshells
    • Named Pipes and Process Substitution
  • The Unix Philosophy Revisited
• 8. A Rapid Introduction to the R Language
  • Getting Started with R and RStudio
  • R Language Basics
    • Simple Calculations in R, Calling Functions, and Getting Help in R
    • Variables and Assignment
    • Vectors, Vectorization, and Indexing
      • Vector types
      • Factors and classes in R
  • Working with and Visualizing Data in R
    • Loading Data into R
    • Exploring and Transforming Dataframes
    • Exploring Data Through Slicing and Dicing: Subsetting Dataframes
    • Exploring Data Visually with ggplot2 I: Scatterplots and Densities
    • Exploring Data Visually with ggplot2 II: Smoothing
    • Binning Data with cut() and Bar Plots with ggplot2
    • Merging and Combining Data: Matching Vectors and Merging Dataframes
    • Using ggplot2 Facets
    • More R Data Structures: Lists
    • Writing and Applying Functions to Lists with lapply() and sapply()
      • Using lapply()
      • Writing functions
      • Digression: Debugging R Code
      • More list apply functions: sapply() and mapply()
    • Working with the Split-Apply-Combine Pattern
    • Exploring Dataframes with dplyr
    • Working with Strings
  • Developing Workflows with R Scripts
    • Control Flow: if, for, and while
    • Working with R Scripts
    • Workflows for Loading and Combining Multiple Files
    • Exporting Data
  • Further R Directions and Resources
• 9. Working with Range Data
  • A Crash Course in Genomic Ranges and Coordinate Systems
  • An Interactive Introduction to Range Data with GenomicRanges
    • Installing and Working with Bioconductor Packages
    • Storing Generic Ranges with IRanges
    • Basic Range Operations: Arithmetic, Transformations, and Set Operations
    • Finding Overlapping Ranges
    • Finding Nearest Ranges and Calculating Distance
    • Run Length Encoding and Views
      • Run-length encoding and coverage()
      • Going from run-length encoded sequences to ranges with slice()
      • Advanced IRanges: Views
    • Storing Genomic Ranges with GenomicRanges
    • Grouping Data with GRangesList
    • Working with Annotation Data: GenomicFeatures and rtracklayer
    • Retrieving Promoter Regions: Flank and Promoters
    • Retrieving Promoter Sequence: Connection GenomicRanges with Sequence Data
    • Getting Intergenic and Intronic Regions: Gaps, Reduce, and Setdiffs in Practice
    • Finding and Working with Overlapping Ranges
    • Calculating Coverage of GRanges Objects
  • Working with Ranges Data on the Command Line with BEDTools
    • Computing Overlaps with BEDTools Intersect
    • BEDTools Slop and Flank
    • Coverage with BEDTools
    • Other BEDTools Subcommands and pybedtools
• 10. Working with Sequence Data
  • The FASTA Format
  • The FASTQ Format
  • Nucleotide Codes
  • Base Qualities
  • Example: Inspecting and Trimming Low-Quality Bases
  • A FASTA/FASTQ Parsing Example: Counting Nucleotides
  • Indexed FASTA Files
• 11. Working with Alignment Data
  • Getting to Know Alignment Formats: SAM and BAM
    • The SAM Header
    • The SAM Alignment Section
    • Bitwise Flags
    • CIGAR Strings
    • Mapping Qualities
  • Command-Line Tools for Working with Alignments in the SAM Format
    • Using samtools view to Convert between SAM and BAM
    • Samtools Sort and Index
    • Extracting and Filtering Alignments with samtools view
      • Extracting alignments from a region with samtools view
      • Filtering alignments with samtools view
  • Visualizing Alignments with samtools tview and the Integrated Genomics Viewer
    • Pileups with samtools pileup, Variant Calling, and Base Alignment Quality
  • Creating Your Own SAM/BAM Processing Tools with Pysam
    • Opening BAM Files, Fetching Alignments from a Region, and Iterating Across Reads
    • Extracting SAM/BAM Header Information from an AlignmentFile Object
    • Working with AlignedSegment Objects
    • Writing a Program to Record Alignment Statistics
    • Additional Pysam Features and Other SAM/BAM APIs
• 12. Bioinformatics Shell Scripting, Writing Pipelines, and Parallelizing Tasks
  • Basic Bash Scripting
    • Writing and Running Robust Bash Scripts
      • A robust Bash header
      • Running Bash scripts
    • Variables and Command Arguments
      • Command-line arguments
    • Conditionals in a Bash Script: if Statements
    • Processing Files with Bash Using for Loops and Globbing
  • Automating File-Processing with find and xargs
    • Using find and xargs
    • Finding Files with find
    • finds Expressions
    • finds -exec: Running Commands on finds Results
    • xargs: A Unix Powertool
    • Using xargs with Replacement Strings to Apply Commands to Files
    • xargs and Parallelization
  • Make and Makefiles: Another Option for Pipelines
• 13. Out-of-Memory Approaches: Tabix and SQLite
  • Fast Access to Indexed Tab-Delimited Files with BGZF and Tabix
    • Compressing Files for Tabix with Bgzip
    • Indexing Files with Tabix
    • Using Tabix
  • Introducing Relational Databases Through SQLite
    • When to Use Relational Databases in Bioinformatics
    • Installing SQLite
    • Exploring SQLite Databases with the Command-Line Interface
    • Querying Out Data: The Almighty SELECT Command
      • Limiting results with LIMIT
      • Selecting columns with SELECT
      • Ordering rows with ORDER BY
      • Filtering which rows with WHERE
    • SQLite Functions
    • SQLite Aggregate Functions
      • Grouping rows with GROUP BY
    • Subqueries
    • Organizing Relational Databases and Joins
      • Organizing relational databases
      • Inner joins
      • Left outer joins
    • Writing to Databases
      • Creating tables
      • Inserting records into tables
      • Indexing
    • Dropping Tables and Deleting Databases
    • Interacting with SQLite from Python
      • Connecting to SQLite databases and creating tables from Python
      • Loading data into a table from Python
    • Dumping Databases
• 14. Conclusion
  • Where to Go From Here?
• Glossary
• Bibliography
• Index

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